Lab Meeting Schedule

The Adams lab gathers once a week for an hour and a half, during which we dissect primary research articles in a journal club format, students present their recent experimental data to the group, and/or protocols for research techniques are discussed.

Summer 2020 Lab Meetings – Fridays, 1:00pm-2:30pm

Date	Presenter	Topic
5/29/20	Ken	Chalk talk – Integrating ApoE, Alzheimer’s Disease, & Fundamentals of Cell and Molecular Biology part 1
6/5/20	Ken	Chalk talk – Integrating ApoE, Alzheimer’s Disease, & Fundamentals of Cell and Molecular Biology part 2
6/12/20	N/A	CANCELED
6/19/20	Ken	Chalk talk – Integrating ApoE, Alzheimer’s Disease, & Fundamentals of Cell and Molecular Biology part 3
6/26/20	Gordo	Zhao et al. (2020) Neuron. Alzheimer’s Risk Factors Age, APOE Genotype, and Sex Drive Distinct Molecular Pathways
7/3/20	N/A	No meeting — Happy 4th!
7/10/20	N/A	CANCELED
7/17/20	Ken	Chalk talk – Molecular Tools & Techniques
7/24/20	Ken	Chalk talk – Molecular Tools & Techniques
7/31/20	Michaela	Blanchard et al. (2020) Nature Medicine. Reconstruction of the human blood–brain barrier in vitro reveals a pathogenic mechanism of APOE4 in pericytes
8/7/20	Adele	Kanaan et al. (2020) Nature Communications. Liquid-liquid phase separation induces pathogenic tau conformations in vitro
8/14/20	TBD	TBD
8/21/20	TBD	TBD
8/28/20	TBD	TBD

Spring 2020 Lab Meetings – Thursdays, 10:00am-11:30am (as of 4/2/2020)

Date	Presenter	Topic
1/29/20	Ken	Update on Lab Projects & Strategy for Spring 2020
2/5/20	Ken	Update on ASBMB & Grant projects
2/12/20	Ken	Data Talk
2/19/20	Adam	Munoz et al. (2018) Journal of Biological Chemistry. The serine protease HtrA1 contributes to the formation of an extracellular 25-kDa apolipoprotein E fragment that stimulates neuritogenesis
2/26/20	Gordo	Eftekharzadeh et al. (2018) Neuron. Tau Protein Disrupts Nucleocytoplasmic Transport in Alzheimer’s Disease
3/4/20	CANCELLED – Spring Break
3/11/20	CANCELLED
3/18/20	CANCELLED
3/25/20	CANCELLED
4/2/20	Ken	Wang et al. (2018) Nature Medicine. Gain of toxic apolipoprotein E4 effects in human iPSC-derived neurons is ameliorated by a small-molecule structure corrector
4/9/20	Adele	Shi et al. (2017) Nature. ApoE4 markedly exacerbates tau-mediated neurodegeneration in a mouse model of tauopathy
4/16/20	Michaela	Rauch et al. (2020) Nature. LRP1 is a master regulator of tau uptake and spread
4/23/20	Michaela	Cont’d: Rauch et al. (2020) Nature. LRP1 is a master regulator of tau uptake and spread
4/30/20	Sarina	Yeh et al. (2016) Neuron. TREM2 binds to apolipoproteins, including APOE and CLU/APOJ, and thereby facilitates uptake of amyloid-beta by microglia

Fall 2019 Lab Meetings – Wednesdays, 4:30pm-6:00pm

Date	Presenter	Topic
9/11/19	Ken	Update on Lab Projects and Strategy for Fall 2019
9/18/19	Adele & Rob	Data talk
9/25/19	Gordo	Boija et al. (2018) Cell. Transcription factors activate genes through phase separation capacity of their activation domains
10/2/19	Adele, Rob, & Gordo	Practice FISH talks
10/9/19	All	Group discussion projects status and strategies for moving forward
10/16/19	Gordo, Michaela, Adam	Data talk
10/23/19	Adele	Shrinivas et al. (2019) Molecular Cell. Enhancer Features that Drive Formation of Transcriptional Condensates
10/30/19	CANCELLED	N/A
11/6/19	CANCELLED	N/A
11/13/19	All	Project updates & ASBMB abstracts submission
11/20/19	Rob	Kilic et al. (2019) EMBO Journal. Phase separation of 53BP1 determines liquid-like behavior of DNA repair compartments
11/27/19	CANCELLED	N/A
12/4/19	Michaela	Sevetson et al. (2000) Journal of Biological Chemistry. A Novel Activation Function for NAB Proteins in EGR-dependent Transcription of the Luteinizing Hormone beta Gene
12/11/19	Michaela	Conclude presentation on Sevetson et al. (2000)

Spring 2019 Lab Meetings – Thursdays, 11:00am-12:30pm

Date	Presenter	Topic
1/24/19	Ken	Review of current Adams lab projects
1/31/19
2/7/19	Ken	Protocol review – Cell splitting
2/14/19	Ken	Protocol review – Cell counting
2/21/19	Everyone	Srinivasan et al. (2006) NAB2 represses transcription by interacting with the CHD4 subunit of the nucleosome remodeling and deacetylase (NuRD) complex. J Biol Chem. 281(22):15129-37. Techniques review embedded within the journal club discussion.
2/28/19
3/7/19
3/14/19	No Meeting – Spring Break
3/21/19	Gordo	Mullenbrock et al. (2011) Global expression analysis identified a preferentially NGF-induced transcriptional program regulated by sustained ERK and AP-1 protein activation during PC12 cell differentiation. J Biol Chem. 286(52):45131-45.
3/28/19	Adele	Adams et al. (2017) Role for Egr1 in the Transcriptional Program Associated with Neuronal Differentiation of PC12 Cells. PLoS ONE. 12(1):e0170076.
4/4/19	Ken	Discussion on fundamentals of Alzheimer Disease pathology
4/11/19	Rob	DeVos et al. (2018) Synaptic Tau Seeding Precedes Tau Pathology in Human Alzheimer’s Disease Brain. Front Neurosci. 12:267.
4/18/19	Kelsey & Jenna	Garcia-Gutierrez et al. (2011) The transcription factor Krox20 is an E3 ligase that sumoylates its Nab coregulators. EMBO Rep. 12(10):1018-23.
4/25/19	Sarina	György et al. (2018) CRISPR/Cas9 Mediated Disruption of the Swedish APP Allele as a Therapeutic Approach for Early-Onset Alzheimer’s Disease. Mol Ther Nucl Acids. 11:429-40.
5/2/19	Adam	Wegmann et al. (2018) Tau protein liquid–liquid phase separation can initiate tau aggregation. EMBO J. 37:e98049.