Lab Meeting Schedule

The Adams lab gathers once a week for an hour and a half, during which we dissect primary research articles in a journal club format, students present their recent experimental data to the group, and/or protocols for research techniques are discussed.

Spring 2021 Lab Meetings – Mondays, 6:00pm-7:30pm

2/1/21MorganHuang et al. (2017) Neuron. ApoE2, ApoE3, and ApoE4 Differentially Stimulate APP Transcription and Aβ Secretion
2/8/21ALLDiscussion on lab projects, communication, and coordination
2/15/21Adam Data Talk – Progress in recombinant apoE production and purification
2/22/21Marissa Neuropsychology for Cell & Molecular Biologists
3/1/21MichaelaData Talk – Progress in molecular cloning & SDM to optimize mammalian apoE expression constructs 
3/8/21KenData Talk –  His-apoE cleavage reactions

Fall 2020 Lab Meetings – Thursdays, 6:00pm-7:30pm

9/24/20KenChalk talk – Summary and Status of ApoE Projects
10/1/20MichaelaArboleda-Velasquez et al. (2019) Nature Medicine. Resistance to autosomal dominant Alzheimer’s disease in an APOE3 Christchurch homozygote: a case report
10/8/20N/AGroup discussion on status of AKTA, PC12 GFP neurites, & HEK Abeta-luci/ferase cells
10/15/20MorganNathan et al. (2002) Brain Research. Apolipoprotein E4 inhibits, and apolipoprotein E3 promotes neurite outgrowth in cultured adult mouse cortical neurons through the low-density lipoprotein receptor-related protein
10/22/20AshlynJournal club article TBD

Summer 2020 Lab Meetings – Fridays, 1:00pm-2:30pm

5/29/20KenChalk talk – Integrating ApoE, Alzheimer’s Disease, & Fundamentals of Cell and Molecular Biology part 1
6/5/20KenChalk talk – Integrating ApoE, Alzheimer’s Disease, & Fundamentals of Cell and Molecular Biology part 2
6/19/20KenChalk talk – Integrating ApoE, Alzheimer’s Disease, & Fundamentals of Cell and Molecular Biology part 3
6/26/20GordoZhao et al. (2020) Neuron. Alzheimer’s Risk Factors Age, APOE Genotype, and Sex Drive Distinct Molecular Pathways
7/3/20N/ANo meeting — Happy 4th!
7/17/20KenChalk talk – Molecular Tools & Techniques
7/24/20KenChalk talk – Molecular Tools & Techniques
7/31/20MichaelaBlanchard et al. (2020) Nature Medicine. Reconstruction of the human blood–brain barrier in vitro reveals a pathogenic mechanism of APOE4 in pericytes
8/7/20AdeleKanaan et al. (2020) Nature Communications. Liquid-liquid phase separation induces pathogenic tau conformations in vitro

Spring 2020 Lab Meetings – Thursdays, 10:00am-11:30am (as of 4/2/2020)

1/29/20KenUpdate on Lab Projects & Strategy for Spring 2020
2/5/20KenUpdate on ASBMB & Grant projects
2/12/20KenData Talk
2/19/20AdamMunoz et al. (2018) Journal of Biological ChemistryThe serine protease HtrA1 contributes to the formation of an extracellular 25-kDa apolipoprotein E fragment that stimulates neuritogenesis
2/26/20GordoEftekharzadeh et al. (2018) Neuron. Tau Protein Disrupts Nucleocytoplasmic Transport in Alzheimer’s Disease
3/4/20CANCELLED – Spring Break
4/2/20KenWang et al. (2018) Nature MedicineGain of toxic apolipoprotein E4 effects in human iPSC-derived neurons is ameliorated by a small-molecule structure corrector
4/9/20AdeleShi et al. (2017) NatureApoE4 markedly exacerbates tau-mediated neurodegeneration in a mouse model of tauopathy
4/16/20MichaelaRauch et al. (2020) NatureLRP1 is a master regulator of tau uptake and spread
4/23/20MichaelaCont’d: Rauch et al. (2020) NatureLRP1 is a master regulator of tau uptake and spread
4/30/20SarinaYeh et al. (2016) NeuronTREM2 binds to apolipoproteins, including APOE and CLU/APOJ, and thereby facilitates uptake of amyloid-beta by microglia

Fall 2019 Lab Meetings – Wednesdays, 4:30pm-6:00pm

9/11/19KenUpdate on Lab Projects and Strategy for Fall 2019
9/18/19Adele & RobData talk
9/25/19GordoBoija et al. (2018) Cell. Transcription factors activate genes through phase separation capacity of their activation domains
10/2/19Adele, Rob, & GordoPractice FISH talks
10/9/19AllGroup discussion projects status and strategies for moving forward
10/16/19Gordo, Michaela, AdamData talk
10/23/19AdeleShrinivas et al. (2019) Molecular Cell. Enhancer Features that Drive Formation of Transcriptional Condensates
11/13/19AllProject updates & ASBMB abstracts submission
11/20/19RobKilic et al. (2019) EMBO Journal. Phase separation of 53BP1 determines liquid-like behavior of DNA repair compartments
12/4/19MichaelaSevetson et al. (2000) Journal of Biological Chemistry. A Novel Activation Function for NAB Proteins in EGR-dependent Transcription of the Luteinizing Hormone beta Gene
12/11/19MichaelaConclude presentation on Sevetson et al. (2000)

Spring 2019 Lab Meetings – Thursdays, 11:00am-12:30pm

1/24/19KenReview of current Adams lab projects
2/7/19KenProtocol review – Cell splitting
2/14/19KenProtocol review – Cell counting
2/21/19EveryoneSrinivasan et al. (2006) NAB2 represses transcription by interacting with the CHD4 subunit of the nucleosome remodeling and deacetylase (NuRD) complex. J Biol Chem. 281(22):15129-37.
Techniques review embedded within the journal club discussion.
3/14/19No Meeting – Spring Break
3/21/19GordoMullenbrock et al. (2011) Global expression analysis identified a preferentially NGF-induced transcriptional program regulated by sustained ERK and AP-1 protein activation during PC12 cell differentiation. J Biol Chem. 286(52):45131-45.
3/28/19AdeleAdams et al. (2017) Role for Egr1 in the Transcriptional Program Associated with Neuronal Differentiation of PC12 Cells. PLoS ONE. 12(1):e0170076.
4/4/19KenDiscussion on fundamentals of Alzheimer Disease pathology
4/11/19RobDeVos et al. (2018) Synaptic Tau Seeding Precedes Tau Pathology in Human Alzheimer’s Disease Brain. Front Neurosci. 12:267.
4/18/19Kelsey & JennaGarcia-Gutierrez et al. (2011) The transcription factor Krox20 is an E3 ligase that sumoylates its Nab coregulators. EMBO Rep. 12(10):1018-23.
4/25/19SarinaGyörgy et al. (2018) CRISPR/Cas9 Mediated Disruption of the Swedish APP Allele as a Therapeutic Approach for Early-Onset Alzheimer’s Disease. Mol Ther Nucl Acids. 11:429-40.
5/2/19AdamWegmann et al. (2018) Tau protein liquid–liquid phase separation can initiate tau aggregation. EMBO J. 37:e98049.